Escherichia coli O157 latex test 1×100 tests Oxoid

Escherichia coli O157 latex test 1×100 tests Oxoid

SKU: OX-DR0620M Categorías: ,

Descripción

E.coli O157 Latex Test Kit.

Code: DR0620M. A latex agglutination test for the identification of Escherichia coli serogroup O157.

Introduction:

– Certain strains of Escherichia coli have been implicated in cases of haemorrhagic colitis (HC) and haemolytic ureamic syndrome (HUS).
– It has been shown that these strains produce a vero-cytotoxin (VT).
– The Escherichia coli serotype most frequently isolated from HC and HUS case is O157:H7.
– Isolation of this serotype from a diarrhoeal stool particularly with the presence of blood is indicative of a vero-cytotoxin producing strain.
– The Oxoid Escherichia coli O157 Latex Test will demonstrate by slide agglutination Escherichia coli strains possessing the O157 serogroup antigen.
– The test is best used in conjunction with Sorbitol MacConkey Agar CM813. E.coli O157:H7 strains do not ferment Sorbitol and therefore give colourless colonies on this medium.
– The majority of Escherichia coli isolates do ferment sorbitol and give characteristic pink colonies.
– Sorbitol MacConkey Agar CM0813 should be used as the primary screen.
– Non-Sorbitol fermenting colonies can then be tested with the latex reagents, to determine whether the isolate belongs to the O157 serogroup and therefore a potential VT-producing strain.

Components of the kit:

– DR0621 Test Latex: Consists of blue latex particles sensitised with specific rabbit antibody reactive with the O157 somatic antigen.
Each kit contains sufficient reagent for 100 tests.
– DR0622 Control Latex: Consists of blue latex particles sensitised with pre-immune rabbit globulin. Each kit contains sufficient reagent for 100 tests.
– DR0623 Positive Control Suspension: A suspension of inactivated Escherichia coli O157 cells in buffer. Sufficient for 25 tests.
– DR0624 Negative Control Suspension: A suspension of Escherichia coli O116 cells in buffer. Sufficient for 25 tests.
– DR0500 Reaction Cards: There are 35 disposable reaction cards provided in the kit. Instruction leaflet.